![]() ![]() ![]() Your protocol can be now found in the instrument memory. Select the folder (category) in which you want to store the protocol on the instrument.Select Upload and find the saved protocol.If you transfer the protocol, select Home > Transfer in BindIt Software. You can start the protocol from the BindIt Software or transfer it onto the instrument.Connect your PC to your KingFisher instrument with a USB cable and select Home > Connect in BindIt Software.Save the protocol on your computer in the preferred folder.Make sure it’s for the correct instrument. Scroll to the Protocols section at the bottom and click the protocol you want to download.You’ll see the product page for that kit. Find the kit you want in the Kit, Reagent, and Protocol Selection Tool and click View Protocols.Download the latest BindIt Software here. Make sure you have BindIt 4.1 Software installed on the computer you use with your KingFisher instrument.Torrey Pines Road, La Jolla, CA, 92037, USA. Despite the large rotation of the branches, both junction structures exhibit an antiparallel arrangement of the continuous strands and opposite polarity of the crossover strands.ĭepartment of Chemistry and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 N. ![]() The stacked-X conformation maximizes base-stacking but causes unfavorable repulsion between phosphate groups, whereas the 135 degrees -rotated "crossed" conformation minimizes electrostatic clashes at the expense of reduced base-stacking. Comparison of the two conformers provides insight into the factors contributing to the flexibility of four-way junctions. The structure of the junction differs substantially from the "stacked-X" conformation observed previously, due to a 135 degrees rotation of the branches. Diversity, Equity, Inclusion, and AccessĪ crystal structure of a 108 nucleotide RNA-DNA complex containing a four-way junction was solved at 3.1 A resolution.Citation, Usage, Privacy Policies, Logo.Biologically Interesting Molecule Reference Dictionary (BIRD). ![]()
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